| 质粒名称: | pAG71 BDNF 3'UTR mut |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 12054 |
| 存储实验室: | David Bartel |
|
载体骨架基本信息 |
|
| 载体名称: | pIS2 |
| 载体出品公司: | pIS2源自pRL-SV40（Promega） |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BDNF 3'UTR mut,BDNF,brain-derived neurotrophic factor |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1141 bp |
| 基因突变: | microRNA识别位点突变（种子匹配）：3751-3756和3921-3926和4853-4858。 |
| GenBank编号 | NM_170735 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | EBV rev primer (GTGGTTTGTCCAAACTCATC) |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAG71 BDNF 3'UTR mut was a gift from David Bartel (Addgene plasmid # 12054 ; http://n2t.net/addgene:12054 ; RRID:Addgene_12054) |
| 参考文献部分: | The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, Bartel DP. Science. 2005 Dec 16. 310(5755):1817-21. 10.1126/science.1121158 PubMed 16308420 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAG71 BDNF 3'UTR mut 4866 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAG71 BDNF 3'UTR mut
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4866)
AUTHORS Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB,
Bartel DP
TITLE The widespread impact of mammalian MicroRNAs on mRNA repression and
evolution.
JOURNAL Science. 2005 Dec 16. 310(5755):1817-21.
PUBMED 16308420
REFERENCE 2 (bases 1 to 4866)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4866
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 29..150
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(40..59)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 94..113
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 283..387
/gene="bla"
/label=AmpR promoter
CDS 388..1248
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(606..625)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1419..2007
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1908..1927
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 2136..2493
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2159..2179)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2344..2479
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2406..2425
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 2563..2695
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 2678..2697
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 2740..2758
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2768..3703
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
1 ctagagcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
61 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
121 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
181 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
241 aaaatcgata aggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
1021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
1081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
1141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
1201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
1261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
1321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
1381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
1441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
1501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
1561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
1621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
1681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
1741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
1801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
1861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
1921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
1981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
2041 gccttttgct ggccttttgc tcacatggct cgacagatct gcgcagcacc atggcctgaa
2101 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
2161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
2281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
2341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
2401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
2461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc
2521 tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
2581 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
2641 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
2701 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
2761 agccaccatg acttcgaaag tttatgatcc agaacaaagg aaacggatga taactggtcc
2821 gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta attattatga
2881 ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg cctcttctta
2941 tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta taccagacct
3001 tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac ttgatcatta
3061 caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca tttttgtcgg
3121 ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag ataagatcaa
3181 agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg aatggcctga
3241 tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg ttttggagaa
3301 taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag aaccagaaga
3361 atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc
3421 atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac aaattgttag
3481 gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta ttgaatcgga
3541 cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta atactgaatt
3601 tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata
3661 tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg agctccagtc
3721 atttgcgcac aacttaaaaa gtctgcatta gaatgcttga taatgttgtg gtttgttgcc
3781 gttgccaaga actgaaaaca taaaaagtta aaaaaaataa taaattgcat gctgctttaa
3841 ttgtgaattg ataataaact gtcctctttc agaaaacaga aaaaaaacac acacacacac
3901 aacaaaaatt tgaaccaaaa cattccgttt acattttaga cagtaagtat cttcgttctt
3961 gttagtacta tatctgtttt actgctttta acttctgata gcgttggaat taaaacaatg
4021 tcaaggtgct gttgtcattg ctttactggc ttaggggatg ggggatgggg ggtatatttt
4081 tgtttgtttt gtgttttttt ttcgtttgtt tgttttgttt tttagttccc acagggagta
4141 gagatgggga aagaattcct acaatatata ttctggctga taaaagatac atttgtatgt
4201 tgtgaagatg tttgcaatat cgatcagatg actagaaagt gaataaaaat taaggcaact
4261 gaacaaaaaa atgctcacac tccacatccc gtgatgcacc tcccaggccc cgctcattct
4321 ttgggcgttg gtcagagtaa gctgcttttg acggaaggac ctatgtttgc tcagaacaca
4381 ttctttcccc ccctccccct ctggtctcct ctttgttttg ttttaaggaa gaaaaatcag
4441 ttgcgcgttc tgaaatattt taccactgct gtgaacaagt gaacacattg tgtcacatca
4501 tgacactcgt ataagcatgg agaacagtga ttttttttta gaacagaaaa caacaaaaaa
4561 taaccccaaa atgaagatta ttttttatga ggagtgaaca tttgggtaaa tcatggctaa
4621 gcttaaaaaa aactcatggt gaggcttaac aatgtcttgt aagcaaaagg tagagccctg
4681 tatcaaccca gaaacaccta gatcagaaca ggaatccaca ttgccagtga catgagactg
4741 aacagccaaa tggaggctat gtggagttgg cattgcattt accggcagtg cgggaggaat
4801 ttctgagtgg ccatcccaag gtctaggtgg aggtggggca tggtatttga gagaatgcaa
4861 aacgat
//
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